[Effects of different methanol feeding strategy on hirudin production in high-density fermentation by recombinant Pichia pastoris].

Abstract

Four different methanol feeding modes were evaluated in the hirudin production in high-density fermentation by Pichia pastoris. It was difficult to avoid methanol excessive in the broth with the feeding strategy only based on DO level. On the other hand, the fluctuation in methanol concentration was observed with methanol feeding strategy by off-line gas… (More)

Topics

Cite this paper

@article{Zhou2002EffectsOD, title={[Effects of different methanol feeding strategy on hirudin production in high-density fermentation by recombinant Pichia pastoris].}, author={Xiang-Shan Zhou and Wei-min Fan and Yuan-xing Zhang}, journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology}, year={2002}, volume={18 3}, pages={348-51} }