[Effect of transfected Rv0901 gene on the activity of mice macrophages].

@article{Zhong2007EffectOT,
  title={[Effect of transfected Rv0901 gene on the activity of mice macrophages].},
  author={Qi Zhong and Lang Bao and Zheng-ling Shang and Yu Liu and Bi H Huang and Su-Xia Yao},
  journal={Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition},
  year={2007},
  volume={38 2},
  pages={226-9}
}
OBJECTIVE To test the effect of Rv0901 gene of Mycobacterium tuberculosis on the activity of mice macrophages. METHODS Peritoneal macrophages of mice were isolated and transfected with pcDNA3. 1 or pcDNA3. 1-Rv0901 plasmid DNA, along with the GFP DNA. The effectiveness of the transfection was detected by RT-PCR. The expression of the GFP and the apoptosis ratio of the macrophages were detected by FCM 72 hours after the transfection. The level of nitric oxide and IFN-gamma in cultural… CONTINUE READING