[Effect of recombinant trichosanthin on proliferation of human cevical cancer Caski cells].

@article{Peng2011EffectOR,
  title={[Effect of recombinant trichosanthin on proliferation of human cevical cancer Caski cells].},
  author={Pingping Peng and Liming Huang and Yanlin Wang and Chengcheng You and Weihong Cao and Huamei Song and Hanxing Tan and Yanzhen Wu},
  journal={Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica},
  year={2011},
  volume={36 18},
  pages={2539-42}
}
OBJECTIVE To observe the effects of high expression of recombinant trichosanthin (rTCS) on the cell proliferation and cell cycle of human cervical cancer Caski cells. METHOD Eukaryotic expression plasmid pcDNA3.1(-)/6His-TCS was constracted and stably transfected into Caski cells. RT-PCR,Western-blot were used to select the clones with rTCS high-expressing. Using pcDNA3.1(-)-transfected cells as the control, MTT assay and flowcytometry were used to elucidate the effects of rTCS high… CONTINUE READING