• Corpus ID: 24593595

[Effect of gamma-glutamyl kinase gene knock-out on metabolism in L-arginine-producing strain Corynebacterium crenatum 8-193].

@article{Li2011EffectOG,
  title={[Effect of gamma-glutamyl kinase gene knock-out on metabolism in L-arginine-producing strain Corynebacterium crenatum 8-193].},
  author={Xiaoman Li and Zhi Zhao and Ying-zi Zhang and Yu Wang and Jiu-yuan Ding},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  year={2011},
  volume={51 11},
  pages={
          1476-84
        }
}
OBJECTIVE In order to optimize precursor supply for L-arginine biosynthesis, we constructed a Corynebacterium crenatum 8-193 mutant with gamma-glutamyl kinase gene (proB) in-frame deletion. The effects of proB knock-out on physiological characteristics of the mutant were investigated. METHODS The upstream and downstream fragments of proB were cloned from C. crenatum 8-193 chromosome and ligated to integration vector. The mutant C. crenatum 8-193-deltaproB was obtained by homologous…