[Domestication of suspension CHO cells and its application in the expression of anti-PSMA antibody].

Abstract

OBJECTIVE To domesticate adherent Chinese hamster ovary (CHO) cells into suspension CHO cells (CHO-S) cultured in serum-free medium,and evaluate the application of the CHO-S cells in antibody expression. METHODS Adherent CHO cells were domesticated into CHO-S cells through suspension culture and gradually decreasing the serum concentration, and eventually… (More)

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