[Dibromoethylacetate - a new agent for fixing the unwound regions of DNA. Denaturation maps of mitochondrial DNA from the rat liver].

@article{Shugalii1982DibromoethylacetateA,
  title={[Dibromoethylacetate - a new agent for fixing the unwound regions of DNA. Denaturation maps of mitochondrial DNA from the rat liver].},
  author={A. V. Shugalii and D I Chernyĭ and M V Lichina and A. V. German and Andrei A. Aleksandrov},
  journal={Molekuliarnaia biologiia},
  year={1982},
  volume={16 5},
  pages={956-64}
}
On the basis of the reaction of dibromoethylacetate with adenine and cytosine at the uncoiled regions of DNA it was possible to fix these molten regions (the degree of denaturation was about 16%) for rat liver mitochondrial DNA. Modification of all accessible adenine and cytosine residues in 0.14 M Na-acetate, pH 5.65, was completed at 70-80 degrees C in 15… CONTINUE READING