[Development of PCR assay for detection of Angiostrongylus cantonensis in Pomacea canaliculata].

Abstract

OBJECTIVE To establish a PCR assay for detecting the third-stage larvae of Angiostrongylus cantonensis in Pomacea canaliculata. METHODS Polymerase chain reaction primers were designed by the software Lasergene, based on the specific cDNA of the third-stage larvae of A.cantonensis in Genbank. The total RNA was prepared from the third-stage larvae of A… (More)

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@article{Zhang2006DevelopmentOP, title={[Development of PCR assay for detection of Angiostrongylus cantonensis in Pomacea canaliculata].}, author={Yi Zhang and Xiao-Nong Zhou and He-xiang Liu and Shan Lv and Li-sha Li and Jin-xiang Lin and You-song Li}, journal={Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases}, year={2006}, volume={24 5}, pages={353-5} }