[Developing of a new feeder-free system and characterization of human embryonic stem cell sublines derived in this system under autogenic and allogenic culturing].

@article{Koltsova2012DevelopingOA,
  title={[Developing of a new feeder-free system and characterization of human embryonic stem cell sublines derived in this system under autogenic and allogenic culturing].},
  author={A M Kol'tsova and Irina V. Voronkina and Olga Gordeeva and Valery V. Zenin and Nadya Lifantseva and A. S. Musorina and Larisa V Smagina and T K Iakovleva and G G Polianskaia},
  journal={Tsitologiia},
  year={2012},
  volume={54 8},
  pages={637-51}
}
A new feeder-free culture system for human embryonic stem cells (hESC) was developed. It consist of extracellular matrix proteins synthesized by feeder cells--mesenchymal stem cell line SC5-MSC, which was derived from initial hESC line SC5. The major ECM proteins--fibronectin and laminin--that maintain hESC growth in feeder-free system were identified. An essential component of this system is a SC5-MSC-conditioned medium. Two hESC sublines were derived. The subline SC5-FF was cultured in… CONTINUE READING