[Determination of total plasma homocysteine and other aminothiols by liquid chromatography coupled to the detection by fluorescence].

@article{Jacob1997DeterminationOT,
  title={[Determination of total plasma homocysteine and other aminothiols by liquid chromatography coupled to the detection by fluorescence].},
  author={Nelly Jacob and Legrand Guillaume and Lo{\"i}c Garçon and Marie Jos{\'e} Foglietti},
  journal={Annales de biologie clinique},
  year={1997},
  volume={55 6},
  pages={
          583-91
        }
}
Plasma concentrations of homocysteine, cysteine, cysteinylglycine and glutathione were measured using a HPLC technique with fluorescence detection of the derivatives obtained with 7-fluoro-2,1,3-benzoxadiazole-4-sulfonamide (ABD-F). Blood was drawn into chilled EDTA-evacuated tubes. After centrifugation at 4 degrees C without delay, plasma samples were kept frozen at -20 degrees C until analysis. Reduction of protein bound aminothiols and disulfides standards was achieved with tri-n… CONTINUE READING