[Determination of jervine and veratramine in Veratrum plants using high performance liquid chromatography coupled with evaporative light scattering detection].

@article{Zhang2008DeterminationOJ,
  title={[Determination of jervine and veratramine in Veratrum plants using high performance liquid chromatography coupled with evaporative light scattering detection].},
  author={Sheng Yuan Zhang and Jianxia Zhou and Qingyao Shou and Ying Hui Peng and Zhengwu Shen},
  journal={Se pu = Chinese journal of chromatography},
  year={2008},
  volume={26 1},
  pages={
          56-9
        }
}
A method of reversed-phase high performance liquid chromatography (HPLC) coupled with evaporative light scattering detection (ELSD) was developed for the determination of jervine and veratramine in veratrum plants. The extraction method of total active alkaloids from the raw material was also established. The separation and quantification were achieved using a Kromasil C8column (250 mm x 4.6 mm, 5 microm), and a mobile phase of acetonitrile and 0.1% trifluoroacetic acid with the following… CONTINUE READING