[Determination of hydroxyproline in rat kidney tissue by HPLC with pre-column derivatization].

Abstract

OBJECTIVE To establish a pre-column derivatization HPLC method for quantitation of hydroxyproline (Hyp) in rat kidney tissue by reversed-phase assay. METHODS Rat kidney samples were hydrolyzed in 6 mol x L⁻¹ HCl under 110 degree for 24 h, then 9-fluorenylmethyl chloroformate (FMOC) was added for the pre-column reaction. The HPLC analysis was performed on… (More)

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