[Determination of anti-native DNA antibodies by immunofluorescence on Crithidia luciliae. Correlation between renal involvement and the presence of high-avidity anti-native DNA antibodies in lupic disease].
Low avidity native DNA - anti-native DNA complexes dissociate at alkaline pH and in high molarity solutions. This property was used to modify the indirect immunofluorescence reaction on Crithidia luciliae in order to assay high avidity anti-native DNA antibodies resistant to alkaline and strongly saline solutions. Assays were performed in the sera of 36… (More)