[Detection of human immunodeficiency virus antigen both free and in immune complexes].

@article{Banfi1995DetectionOH,
  title={[Detection of human immunodeficiency virus antigen both free and in immune complexes].},
  author={N H Banfi and M V Minervini and Adriana E Scoccia},
  journal={Revista Argentina de microbiologia},
  year={1995},
  volume={27 3},
  pages={123-9}
}
The aim of this work was to increase sensitivity in the detection of antigens from HIV-infected patients, through a process of immune complex dissociation without loss of antigenicity. 500 microliters of sera were mixed with 100 microliters of PEG 12%, stored one night in refrigerator, and centrifuged at 2000 g during 20 minutes. 200 microliters of buffer AcH/Ac- (pH 3.5) were added to the sediment, and incubated at 37 degrees C during one hour with periodic shaking. This was neutralized with… CONTINUE READING
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