[Detection of Aspergillus fumigatus DNA by polymerase chain reaction in the clinical samples from individuals with pulmonary aspergillosis].

@article{Nakamura1994DetectionOA,
  title={[Detection of Aspergillus fumigatus DNA by polymerase chain reaction in the clinical samples from individuals with pulmonary aspergillosis].},
  author={Hiroshi Nakamura and Yoichi Shibata and Yoshikuni Kudo and Shoji Saito and Humihiko Kimura and Hitonobu Tomoike},
  journal={Rinsho byori. The Japanese journal of clinical pathology},
  year={1994},
  volume={42 7},
  pages={676-81}
}
Aspergillosis is a disastrous cause of mortality and morbidity in patients in an immunocompromised state and old lung tuberculosis. However, the diagnosis of aspergillosis remains unsettled. In the present study, we developed a test to detect the Aspergillus fumigatus DNA using polymerase chain reaction (PCR) in clinical samples including sputum and bronchial aspirations from the patients with aspergillosis. We selected 2 pairs of oligonucleotide primers and the DNA for the small subunit… CONTINUE READING