[Detection and identification method of three mycobacterium species and genus specific detection by polymerase chain reaction and DNA hybridization with alkaline phosphatase labeled oligonucleotide probe].

@article{Komatsu1996DetectionAI,
  title={[Detection and identification method of three mycobacterium species and genus specific detection by polymerase chain reaction and DNA hybridization with alkaline phosphatase labeled oligonucleotide probe].},
  author={M. Komatsu and K. Shimakawa and M. Aihara and S. Matsuo and T. Ezaki},
  journal={Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases},
  year={1996},
  volume={70 2},
  pages={
          141-50
        }
}
  • M. Komatsu, K. Shimakawa, +2 authors T. Ezaki
  • Published 1996
  • Medicine
  • Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Rapid detection and identification method of mycobacteria from specimen with infected mycobacteriosis was established by the combination of polymerase chain reaction and alkaline phosphatase labeled oligonucleotide probe (PCR-ALPDH). We prepaired four kinds of probes: Mycobacterium tuberculosis complex, M. avium, M. intracellulare, and mycobacterial-probe other than tuberculosis. PCR-ALPDH was compared with conventional methods using 234 specimens which suspected mycobacteriosis. In culture, 68… Expand
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