[Decreased chaperone activity of alpha-crystallin by carbamylation in vitro].

PURPOSE To investigate the effort of carbamylation, an important post-translational modification of lens proteins, on chaperone activity of alpha-crystallin. METHODS The alphaL-crystallin and betaL-crystallin were isolated from bovine lens. Carbamylation was performed by different concentrations of potassium [14C] cyanate to alphaL-crystallin at 37… CONTINUE READING