[De novo design, non-chromatographic purification and salt-effect of elastin-like polypeptides].

@article{Huang2011DeND,
  title={[De novo design, non-chromatographic purification and salt-effect of elastin-like polypeptides].},
  author={Kaizong Huang and Jingjing Li and Wei Li and Huihua Ge and Wenyan Wang and Guangya Zhang},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2011},
  volume={27 4},
  pages={653-8}
}
Elastin-like polypeptides (ELPs) are temperature sensitive biopolymers composed of a Val-Pro-Gly-Xaa-Gly pentapeptide repeat that derived from a structural motif found in mammalian elastin. It was a promising tag for recombinant protein purification. Here, we de novo designed a novel ELPs gene and cloned it into the modified expression vector pET-22b… CONTINUE READING