[Cytoplasmic expression of VP1 gene of coxsackievirus B3].

Abstract

OBJECTIVE To increase the immune effect of gene vaccine, T7 RNA polymerase was used to establish a system of cytoplasmic expression. METHODS (1) The plasmid pT7 EMCVP1, including T7 promoter sequence, 5'-untranslated sequence of encephalomyocarditis (EMC) virus, VP1 sequence of coxsackievirus B3 (CVB3), was cotransfected with the plasmid pAR 3132, which… (More)

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