[Culture optimization for protein expression in Escherichia coli].

Abstract

OBJECTIVE We developed a new culture-complex auto-inducing media (CAI) for heterogenous protein expression in Escherichia coli. METHODS To test expression efficiency in the CAI, we constructed seven different plasmids named p-1, p-2, p-3, p-4, p-5, p-6 and p-7. These plasmids were transformed into E. coli BL21, then expressed in both Luria-Bertani madia… (More)

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@article{Xu2009CultureOF, title={[Culture optimization for protein expression in Escherichia coli].}, author={Linglong Xu and Xingming Shi and Yunfeng Wang and Yan Sun and Mei Wang}, journal={Wei sheng wu xue bao = Acta microbiologica Sinica}, year={2009}, volume={49 1}, pages={128-34} }