[Construction of the eukaryotic expression vector of bioactive fragment of human bactericidal/permeability-increasing protein and its expression in CHO cells].

Abstract

AIM To construct the eukaryotic expression vector of the cDNA sequence encoding bioactive N-terminal fragment of human bactericidal/permeability-increasing protein (BPI) and express it in CHO cells. METHODS Total RNA was extracted from human polymorphonuclear leukocytes (PMN) and subjected to reverse transcription, then the human BPI cDNA gene was… (More)

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