[Construction of shRNA expression vectors for autophagy gene Beclin 1 and effect of the vectors on transfected HeLa cells in vitro].


OBJECTIVE Autophagy gene Beclin 1 plays an important role in several types of human cancer. In this study, RNA interference (RNAi) technique was employed to determine the effect of inhibiting Beclin 1 on the growth of tumor cells. METHODS According to the encoding sequence of mRNA of Beclin 1, the target site for the RNAi technique was designed and the… (More)


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