[Construction of recombinant rat δNδC/VEGF-C/Cys152Ser retrovirus vector and its expression in RAW 264.7 cells].

AIM To construct a retroviral vector containing rat δNδC/VEGFand verify its expression in RAW 264.7 cells. METHODS The ddVEGF-C gene was amplified by polymerase chain reaction (PCR) from pSecTag-ddVEGF-C and cloned into pLPCX vector. After the procedure of PCR, double enzyme digestion analysis and DNA sequencing, the recombinant plasmid was transfected… (More)