Corpus ID: 202702156

[Construction of recombinant Bb(pGEX-OprF-I) vaccine of Pseudomonas aeruginosa and its protection elicited in mice].

@article{Liang2019ConstructionOR,
  title={[Construction of recombinant Bb(pGEX-OprF-I) vaccine of Pseudomonas aeruginosa and its protection elicited in mice].},
  author={Cheng Liang and Wengui Li},
  journal={Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology},
  year={2019},
  volume={35 7},
  pages={
          589-594
        }
}
  • Cheng Liang, Wengui Li
  • Published 2019
  • Biology, Medicine
  • Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Objective To construct and identify Bifidobacterium bifidum-vectored outer membrane protein F-I[rBb(pGEX-OprF-I)] vaccine of Pseudomonas aeruginosa and observe its protection against Pseudomonas aeruginosa infection in mice. Methods OprF and OprI genes were amplified by PCR, then the OprF-I fusion gene obtained by gene SOEing was digested and ligated into the vector pGEX-1λT to construct the recombinant plasmid pGEX-OprF-I. The plasmid was transformed into Bifidobacterium bifidum (Bb) by… Expand