[Construction of novel recombinant Escherichia coli capable of producing 1,3-propanediol].

@article{Zhang2005ConstructionON,
  title={[Construction of novel recombinant Escherichia coli capable of producing 1,3-propanediol].},
  author={Xiao-mei Zhang and Xue-Ming Tang and Bin Zhuge and Wei Shen and Zhi-ming Rao and Hui-ying Fang and Jian Zhuge},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2005},
  volume={21 5},
  pages={
          743-7
        }
}
The 1,3-propanediol oxidoreductase isoenzyme encoding gene (yqhD) from E. coli was amplified by PCR. yqhD was inserted in pEtac to yield the recombinant expression vector pEtac-yqhD. Over-expression of yqhD in E. coli JM109 was achieved with pEtac-yqhD. SDS-PAGE analysis showed an over-expressed recombinant product at about 43 kD, consistent with the molecular weight predicted from gene sequence. Compared with E. coli JM109 (pEtac), the 1,3-propanediol oxidoreductase isoenzyme activity of the… CONTINUE READING