[Construction of eukaryotic expression vector and package of lentivirus vector encoding human leukocyte antigen-G5].

Abstract

AIM To construct a lentiviral expression vector carrying HLA-G5. METHODS The CDs region of HLA-G5 gene was cloned into the lentiviral vector by restriction endonuclease Nhe I/Not I digestion and T4DNA ligase ligation. After transformation into completent E.coli cells, the candidate clones were identified by PCR and kidney cell line 293T cells by… (More)

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