[Construction of cyclin D1 recombinant plasmids and its expression in human nasopharyngeal carcinoma cells].

Abstract

OBJECTIVE To construct the eukaryotic expression vectors of human cyclin D1 gene and express them in poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z cells). METHODS The full-length cyclin D1 was cloned from CNE-2Z cells by RT-PCR. The cDNA fragments were inserted into pIRES2-EGFP plasmids and pEGFP-C2 plasmids and confirmed by restriction… (More)

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