[Construction of cDNA library of 'Hongyang' kiwifruit and analysis of F3H expression].

@article{Yang2009ConstructionOC,
  title={[Construction of cDNA library of 'Hongyang' kiwifruit and analysis of F3H expression].},
  author={Hong-li Yang and Yan-Chang Wang and Zheng-Wang Jiang and Hong-Wen Huang},
  journal={Yi chuan = Hereditas},
  year={2009},
  volume={31 12},
  pages={1265-72}
}
The SMART switching mechanism at 5' end of the RNA transcript technique was used to construct a cDNA library from inner pericarp of the red flesh kiwifruit Actinidia chinesis cv 'Hongyang'. Construction of cDNA library facilitated cloning of the genes associated with the secondary metabolism, the specific genes in the course of anthocyanin biosynthesis. The titers of the primary library and the amplified library were 6.7x104 cfu/mL and 2.72x108 cfu/mL, respectively. The recom-bination rate was… CONTINUE READING