[Construction of a yeast model for screening Aedes albopictus ecdysone agonist pesticides].

Abstract

OBJECTIVE To reconstitute a transactivation system in yeast (yeast model) for screening the pesticides acting on ecdysone metabolism route and eventually influencing the process of ecdysis. METHODS The fragment of 5 times repeated EcRE from Drosophila melanogaster was synthesized and the HSP27 promoter from D. melanogaster genome was amplified with PCR… (More)

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