[Construction of a human factor VIII gene-containing plasmid and its expression in Cos-7 cells].

Abstract

OBJECTIVE To construct an eukaryotic expressing plasmid--pAd CMV Link F VIII DB and express it in Cos-7 cells. METHODS An eukaryotic expressing plasmid--pAd CMV Link F VIII DB was constructed by inserting human factor VIII cDNA (F VIII DB, 4.6 kb), in which most part of B domain was deleted, into an adenovirus sequence-containing plasmid, and then Cos-7… (More)

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