[Construction of NF-kappaB siRNA expression vector].

  title={[Construction of NF-kappaB siRNA expression vector].},
  author={Yunusi Kurexi and Guo-hong Huang and Mei Long and Jie He and Maimaitiaili Nasier and Yan-ping Xia and Wufuer Hamulati},
  journal={Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences},
  volume={32 5},
OBJECTIVE To construct a NF-kappaB siRNA expression vector and to detect the specific silencing effect of the siRNA on the expression of NF-kappaB protein. METHODS pcDNA3.1/CT-GFP-TOPO recombinant eukaryotic expression vector and pSilencer 1.0-U6-siRNA-NF-kappaB recombinant vector were constructed respectively. These 2 recombination plasmids were co-transfected into COS-7 cells, and the NF-kappaB silence induced by RNAi was detected by Western blot and the inverted fluorescence microscope… CONTINUE READING

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