Corpus ID: 29830589

[Construction of AML1-ETO eukaryotic expression vector and its effects on proliferation and differentiation of U937 cells].

@article{Zhuang2011ConstructionOA,
  title={[Construction of AML1-ETO eukaryotic expression vector and its effects on proliferation and differentiation of U937 cells].},
  author={Wen-yue Zhuang and Zi X. Chen and Xiao-fei Qi and Jian-nong Cen and Hong-jie Shen and Yun Zhao},
  journal={Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi},
  year={2011},
  volume={32 6},
  pages={
          373-7
        }
}
OBJECTIVE To construct a pcDNA3.1-AML1-ETO expression vector and investigate its effects on proliferation and differentiation of U937 leukemic cells. METHODS AML1-ETO gene was amplified by PCR from pCMV5-AML1-ETO and inserted into eukaryotic expression plasmid pcDNA3.1/V5-His-TOPO. The recombinant plasmid was transfected into U937 cells by Lipofectamin 2000. Individual clones selected with G418 were isolated. The integration and the expression levels of AML1-ETO in transfectants were… Expand