[Construction and identification of matrix metalloproteinase inhibitor-1 siRNA eukaryotic expression vectors].

@article{Tian2007ConstructionAI,
  title={[Construction and identification of matrix metalloproteinase inhibitor-1 siRNA eukaryotic expression vectors].},
  author={Li Li Tian and Ji-chang Li and Guoqiang Zhao and Kui-Sheng Chen and Wen-hui Fan and X. Z. Lou and Miao-miao Sun and Xue-quan Cao},
  journal={Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology},
  year={2007},
  volume={15 3},
  pages={
          196-8
        }
}
OBJECTIVE To construct TIMP-1 siRNA eukaryotic expression vectors and evaluate their effect on TIMP-1 mRNA expression in hepatic stellate cells. METHODS The combinant lone DNA with cutting sites of BamH I and Xho I enzyme according to the sequences of 447-465, 552-540 TIMP-1 of rats and nonspecific sequence were selected and cloned to pGEM-T vector and sub-cloned to pRNAT-U6.2. They were then identified by double enzyme digestion analysis and DNA sequencing. Three plasmids were transfected… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…