[Construction and identification of SPARC shRNA lentiviral vector and its expression in human SKM-1 cells].


AIM To construct a lentiviral vector expressing small-hairpin RNA(shRNA) targeting SPARC gene and investigate its silenced effect on SPARC in human myelodysplastic syndromes(MDS) cell line SKM-1. METHODS The targeting sequence of SPARC gene which can be effectively silenced in RNA interference was confirmed in our previous study. The designed and… (More)


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