[Construction and identification of SPARC shRNA lentiviral vector and its expression in human SKM-1 cells].

Abstract

AIM To construct a lentiviral vector expressing small-hairpin RNA(shRNA) targeting SPARC gene and investigate its silenced effect on SPARC in human myelodysplastic syndromes(MDS) cell line SKM-1. METHODS The targeting sequence of SPARC gene which can be effectively silenced in RNA interference was confirmed in our previous study. The designed and… (More)

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.