• Biology, Medicine
  • Published in
    Sheng wu gong cheng xue bao…
    2005

[Construction and expression of single chain variable fragments (ScFv) against human CD19 antigen].

@article{Chen2005ConstructionAE,
  title={[Construction and expression of single chain variable fragments (ScFv) against human CD19 antigen].},
  author={Sen Chen and Qing Rao and Jian-xiang Wang and Min Wang},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2005},
  volume={21 5},
  pages={
          686-91
        }
}
The genes encoding for the light and heavy chain variable regions were cloned by RT-PCR from a murine monoclonal hybridoma cell line, which could produce monoclonal antibody to recognize CD19 antigen on human B lymphocyte. Then fused the light and heavy chain variable regions together by a short peptide linker containing 15 amino acid (Gly4Ser)3 using splice-overlap extensive PCR. The recombinant anti-CD19- ScFv was subcloned into the expression vector pET28a and induced to be expressed by IPTG… CONTINUE READING