[Construction and expression of recombinant adenovirus expression vector of beta-defensin-2].

Abstract

OBJECTIVE To explore the possibility of stable expression of cationic peptide beta-defensin-2 in eukaryocytes with adenovirus vector. METHODS The rat beta-defensin-2 (rBD2) gene was cloned at the downstream of CMV promoter of the adenoviral shuttle plasmid pShuttle-CMV. Then pShuttle-CMV-rBD2 was transformed into E. coli BJ5183-AD-1, in which… (More)

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@article{Shi2006ConstructionAE, title={[Construction and expression of recombinant adenovirus expression vector of beta-defensin-2].}, author={Zhuo Shi and Qiang Shu and Zheng-Yan Zhao}, journal={Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences}, year={2006}, volume={35 6}, pages={590-5} }