[Construction and expression of fusion gene encoding Hyper-IL-6 in mammalian cells].

@article{Qie2006ConstructionAE,
  title={[Construction and expression of fusion gene encoding Hyper-IL-6 in mammalian cells].},
  author={Chun-hua Qie and Bo Qin and Ai-long Huang and Qi Liu and Guo-min Chen},
  journal={Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology},
  year={2006},
  volume={22 2},
  pages={141-3, 147}
}
AIM To construct and express fusion gene encoding Hyper-IL-6 in mammalian cells. METHODS Using geneSOEing method, human sIL-6R cDNA was fused with IL-6 cDNA by a linker rich in glycine through PCR technique and cloned into pIRES(2)-EGFP. The constructed plasmid was transfected into mammalian cells. The expression of fusion gene encoding Hyper-IL-6 was… CONTINUE READING