[Construction and expression of eukaryotic expression vector for pEGFP-GPC3].

AIM To construct and expression of eukaryotic expression vector of glypican-3 (GPC3) pEGFP-GPC3 in mouse dendritic cells (DCs). METHODS The target gene fragment of GPC3 and empty vector were obtained from plasmid pCMV-SPORT6-GPC3 and vector pEGFP-N1 digested by double enzymes. GPC3 and empty vector were linked by ligase T4, and then transfected into E… CONTINUE READING