[Construction and expression of a prokaryotic expression plasmid of idiotypic vaccine against B cell lymphoma: encoding the fusion genes of single-chain variable fragment and MCP-3].

Abstract

The aim was to construct a prokaryotic expression plasmid encoding the fusion gene of single-chain variable fragment and monocyte chemotactic protein-3 (MCP-3). The cDNAs of immunoglobulin (Ig) VH and Ig VL were amplified by RT-PCR and assembled into the single-chain variable fragment (scFv) by recombinant PCR method. The cDNAs of Ig VH and Ig VL were… (More)

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Cite this paper

@article{Wang2006ConstructionAE, title={[Construction and expression of a prokaryotic expression plasmid of idiotypic vaccine against B cell lymphoma: encoding the fusion genes of single-chain variable fragment and MCP-3].}, author={Fu-xu Wang and Bing Zhao and Yun-hui Cheng and Ling Pan and Jian-min Luo and Xue-jun Zhang and Zuo-ren Dong}, journal={Zhongguo shi yan xue ye xue za zhi}, year={2006}, volume={14 6}, pages={1151-5} }