[Construction and expression of a fusion gene of human heptoma peptide with human heat shock protein 70].

Abstract

AIM To construct and express a fusion gene of human heptoma peptide (EPVTKAEML) with human heat shock protein 70 (HSP70). METHODS A cDNA fragment encoding EPVTKAEML was added to 3 terminus of human HSP70 gene by PCR amplification. The PCR products of fusion gene were cloned into pET-28a(+)vector. The recombinant plasmid pET-28a(+)/EPVTKAEML-HSP70 was… (More)

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@article{Qu2008ConstructionAE, title={[Construction and expression of a fusion gene of human heptoma peptide with human heat shock protein 70].}, author={Ping Qu and Jia-hai Ma and Yong Huang and Li-bing Liu and Ru Tie and Fang-e Liu and Xiao-Jun Huang}, journal={Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology}, year={2008}, volume={24 2}, pages={113-4} }