[Construction, expression and immunogenicity of eukaryotic vectors based on goat pox virus P32 gene].

Abstract

The full-length P32 gene and the truncated P32 gene (MP-32) were amplified from the recombinant plasmid pMD-P32 by polymerase chain reaction (PCR) and cloned into pcDNA3. 1(+) and pcDNA3.1-CpG respectively. The recombinant plasmids (pcDNA3.1-P32, pcDNA3.1-CpG-P32 and pcDNA3. 1-CpG-MP32) were transfected into BHK-21 cells by using lipofectin. The expressed… (More)

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Cite this paper

@article{Chen2008ConstructionEA, title={[Construction, expression and immunogenicity of eukaryotic vectors based on goat pox virus P32 gene].}, author={Yi-Xia Chen and Xue-peng Cai and Zhi-zhong Jing and Jun-Tao Ding and Ying Wang and Xue-lian Meng and Yan Zhang and Wan-zhong Jia and Jun Qiao and Hong-Bin Yan and Yong-Xiang Fang and Guo-hua Chen and Xue-nong Luo}, journal={Bing du xue bao = Chinese journal of virology}, year={2008}, volume={24 2}, pages={133-7} }