[Construction, expression and immunocompetence of Neisseria gonorrhoeae porB and Escherichia coli itB fusion gene].

@article{Dai2010ConstructionEA,
  title={[Construction, expression and immunocompetence of Neisseria gonorrhoeae porB and Escherichia coli itB fusion gene].},
  author={Zhibing Dai and Sihai Hu and Min Chen and Chunxue Lu and Yufeng Wang and Qingnan Liu and Yukuai Zhang and Minjun Yu and Cuiming Zhu and Zhongyu Li},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  year={2010},
  volume={50 4},
  pages={517-23}
}
OBJECTIVE To construct prokaryotic fusion gene expression vector pET-30a/ltB-porB, express the recombinant fusion protein LTB-PorB and analyze the immunocompetence of the recombinant fusion protein in female BALB/c mice through intranasally immunization. METHODS B subunit of Escherichia coli heat-labile enterotoxin (LTB) and Neisseria gonorrhoeae Porin B (PorB) fusion gene, LTB gene and PorB gene were cloned into prokaryotic vector pET-30a. The recombinants were identified by Polymerase Chain… CONTINUE READING