[Comparison between 3 antigens for the serodiagnosis of heartwater disease by indirect immunofluorescence].

@article{Martinez1990ComparisonB3,
  title={[Comparison between 3 antigens for the serodiagnosis of heartwater disease by indirect immunofluorescence].},
  author={D. Martinez and J W Swinkels and Emmanuel Camus and Frans Jongejan},
  journal={Revue d'elevage et de medecine veterinaire des pays tropicaux},
  year={1990},
  volume={43 2},
  pages={159-66}
}
A cell line of bovine endothelial cells (E5), infected with 3 different stocks of Cowdria ruminantium, was used as antigen in an indirect fluorescent antibody test for the serodiagnosis of heartwater. These antigens were compared to peritoneal macrophages from mice infected with the Kümm stock and to caprine neutrophils in primary cultures from goats infected with 4 different stocks of Cowdria. The use of endothelial cell cultures proved to be superior in all respects. The antigens can be… CONTINUE READING