[Compared D-amino acid oxidase expression in different Pichia pastoris host strains].

@article{Feng2004ComparedDA,
  title={[Compared D-amino acid oxidase expression in different Pichia pastoris host strains].},
  author={Mei-qing Feng and Hai Feng Huang and Xun-long Shi and Z Yu and Zhong-Yi Yuan and Pei Zhou},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2004},
  volume={20 4},
  pages={572-7}
}
To compare the DAAO expression level in different Pichia pastoris host strains, the gene encoding DAAO from Trigonopsis variabilis was cloned into plasmid pPIC3.5k and then transformed into P. pastoris GS115 and KM71 respectively. The positive transformants PDK13 (MutS) and PD27 (Mut+) were obtained by PCR analysis. Their optimal and different expression… CONTINUE READING