[Comparative study of the antigenic and immunogenic properties of native and recombinant Marburg virus VP35 proteins].

@article{Sorokin1999ComparativeSO,
  title={[Comparative study of the antigenic and immunogenic properties of native and recombinant Marburg virus VP35 proteins].},
  author={Alexander V. Sorokin and Elena I Kazachinskaia and Alla V. Kachko and Alla V. Ivanova and A. A. Bukreev and Ivan A Razumov},
  journal={Voprosy virusologii},
  year={1999},
  volume={44 5},
  pages={206-13}
}
Antigenic structure of Marburg virus (MBG) VP35 was compared with that of the recombinant VP35 (f35) expressed in a prokaryotic system. For this purpose, a gene encoding the full-length VP35 was cloned in vector pQE31(QIAGEN) and expressed at about 70 mg/liter culture fluid in Escherichia coli JM103 as a recombinant fusion protein f35. BALB/c mice were immunized with soluble f35 or purified inactivated virions of MBG. Antibodies cross-reacting with VP35 and f35 antigens were detected by ELISA… CONTINUE READING