[Cluster analysis of Dendrobium by RAPD and design of specific primer for Dendrobium candidum].

@article{Zhang2001ClusterAO,
  title={[Cluster analysis of Dendrobium by RAPD and design of specific primer for Dendrobium candidum].},
  author={Ming Zhang and Hao Ran Huang and Sha Mei Liao and Jeff Y. Gao},
  journal={Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica},
  year={2001},
  volume={26 7},
  pages={442-7}
}
OBJECTIVE To analyze the genetic relationship of Dendrobium and design a specific primer to differentiate Dendrobium candidum effectively. METHOD Random amplified polymorphic DNA (RAPD) technique was used to analyze the genetic relationships of 26 species of Dendrobium and one species of Ephemerantha. Dendrogram was constructed by UPGMA. According to the sequence of DNA fragment selected, Sangon 18 primer had been extended from 3' extreme to 20 bp in order to form a specific primer. RESULT… CONTINUE READING