[Cloning of cDNA fragments of human defensins].


In order to acquire the cDNA of a small molecular peptide with biological activity, the cDNA encoding human defensins was isolated from total RNAs of peripheral leukocyte in a patient with chronic myelogenous leukemia by the RT-PCR method, about 280 bp in length. The RT-PCR products were cloned into the pUCm-T vector, and may be used to construct a novel… (More)


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