[Cloning and sequencing of 16S rRNA gene of Phytoplasma CWB1 strain associated with cactus witches' broom].

@article{Cai2001CloningAS,
  title={[Cloning and sequencing of 16S rRNA gene of Phytoplasma CWB1 strain associated with cactus witches' broom].},
  author={Huafang Cai and Fan Li and Byung-Whi Kong and Hairu Chen},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  year={2001},
  volume={41 6},
  pages={
          693-8
        }
}
A 1.5 kb DNA fragment was amplified in DNA samples extracted from Opuntia salmiana porm showed witches'-broom symptom. The result indicates the existence of phytoplasma associated with this disease and this phytoplasma was designated as CWB1. The amplified fragment was ligated to pGEM-T easy vector and then transformed into JM109 strain of E. coli. Cloned DNA fragments were verified by PCR, restriction endonuclease (EcoRI) digestion and sequence analysis. The result revealed that the 16S rRNA… CONTINUE READING