[Cloning and sequence analysis on cDNA of squalene epoxidase gene in Eleutherococcus senticosus].

OBJECTIVE To clone and sequence the cDNA of squalene epoxidase gene in Eleutherococcus senticosus. METHOD Total RNA of E. senticosus was extracted by the improved isothiocyanate method and reverse transcripted into cDNA. The primers were designed depending on the reported SE cDNA sequences of Panax ginseng. The SE cDNAs in E. senticosus was amplified… CONTINUE READING