[Cloning and prokaryotic expression of the gene encoding PGRP domain of mouse long peptidoglycan recognition protein].


OBJECTIVE To clone the gene coding for the peptidoglycan recognition protein (PGRP) domain (PGRPd) of mouse long PGRP (mPGRP-L) and express the protein in E. coli. METHODS The cDNA fragment encoding PGRPd of mPGRP-L was obtained by RT-PCR from the total RNA of Balb/C mouse liver cells and cloned into pUCm-T vector. The recombinant plasmid were identified… (More)


  • Presentations referencing similar topics