[Cloning and expression of urate oxidase and its application in serum uric acid analysis].

Abstract

Anurate oxidase (uricase, EC 1.7.3.3) gene from Candida utilis AS2.117 was cloned by PCR amplification with primers derived from conserved regions of published uicase DNA sequence. The DNA sequence of cloned uricase gene was determined and a high homology compared to the reported gene was found. The cloned gene was inserted into Bam H I and Nde I sites of… (More)

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@article{Zhu2001CloningAE, title={[Cloning and expression of urate oxidase and its application in serum uric acid analysis].}, author={X. J. Zhu and J. G. Liu and Guanbin Li}, journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology}, year={2001}, volume={17 1}, pages={68-72} }